The enteric neural crest progressively loses capacity to form enteric nervous system

D Zhang; BN Rollo; N Nagy; L Stamp; DF Newgreen

Journal article

The enteric neural crest progressively loses capacity to form enteric nervous system

D Zhang, BN Rollo, N Nagy, L Stamp, DF Newgreen

Developmental Biology | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2019

DOI: 10.1016/j.ydbio.2018.11.017

Abstract

Cells of the vagal neural crest (NC) form most of the enteric nervous system (ENS) by a colonising wave in the embryonic gut, with high cell proliferation and differentiation. Enteric neuropathies have an ENS deficit and cell replacement has been suggested as therapy. This would be performed post-natally, which raises the question of whether the ENS cell population retains its initial ENS-forming potential with age. We tested this on the avian model in organ culture in vitro (3 days) using recipient aneural chick midgut/hindgut combined with ENS-donor quail midgut or hindgut of ages QE5 to QE10. ENS cells from young donor tissues (≤ QE6) avidly colonised the aneural recipient, but this capac..

View full abstract

University of Melbourne Researchers

Lincon Stamp Author

Related Projects (2)

CELL THERAPY FOR ENTERIC NEUROPATHIES - THE ESSENTIAL NEXT STEPS

Gastrointestinal motility disorders caused by damage or diseased neurons in the gut wall ('enteric neuropathies) are some of the most clinic..

Understanding interactions between gut epithelial stem cells and neurons

This project aims to investigate the interaction between gut neurons and the epithelial stem cell compartment, as well as the relationship b..

Grants

Awarded by National Institutes of Health

Funding Acknowledgements

We extend thanks to Dr. Craig Smith, MCRI, Melbourne, for rabbit SoxE antibody, Dr. Thomas Muller, Max-Delbruck-Center, Berlin, for the rabbit B-FABP antibody and Dr. Michael Wegner, Emil-Fischer-Zentrum, Erlangen, for the mouse Sox10 antibody. QCPN monoclonal antibody developed by B.M. and J.A. Carlson, was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242. DFN, DZ and BNR were supported by the National Health and Medical Research Council Grants 607379 and 1069757. NN is supported by a Bolyai Fellowship from the Hungarian Academy of Sciences and Hungarian Science Foundation NKFI Grant (124740). LS was supported by the National Health and Medical Research Council Grant 1079234 and Australian Research Council DECRA Fellowship DE180100261. MCRI facilities are supported by the Victorian Government's Operational Infrastructure Support Program.